Growth of high quality protein crystals.
نویسندگان
چکیده
منابع مشابه
Approach for growth of high-quality and large protein crystals
Three crystallization methods for growing large high-quality protein crystals, i.e. crystallization in the presence of a semi-solid agarose gel, top-seeded solution growth (TSSG) and a large-scale hanging-drop method, have previously been presented. In this study the effectiveness of crystallization in the presence of a semi-solid agarose gel has been further evaluated by crystallizing addition...
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High density BaZrO3 ceramic crucibles have been successfully fabricated and used to grow high quality YBa2Cu3O7-x single crystals by the BaO-CuO self flux method. The ceramic crucibles are inert and insoluble in the Y2O3-BaO-CuO melt, have a long lifetime, and can be used repeatedly for crystal growth runs. The crystals grown using the crucibles have not only high chemical purity (99.99-99.995%...
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Protein crystallization is important for structural biology. The rate at which a protein crystallizes is often the bottleneck in determining the protein's structure. Here, we give a physical model for the growth rates of protein crystals. Most materials crystallize faster under stronger growth conditions; however, protein crystallization slows down under the strongest conditions. Proteins requi...
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Precise protein structure determination provides significant information on life science research, although high-quality crystals are not easily obtained. We developed a system for producing high-quality protein crystals with high throughput. Using this system, gravity-controlled crystallization are made possible by a magnetic microgravity environment. In addition, in-situ and real-time observa...
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PURPOSE Interphotoreceptor retinol-binding protein (IRBP), composed of two or four homologous modules in tandem, plays an important role in retinoid trafficking between the retinal pigmented epithelium, photoreceptors, and Müller cells. The exact nature of this role is not yet clear. Attempts to purify the full-length retinal IRBP to homogeneity for crystallization purposes have largely been un...
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ژورنال
عنوان ژورنال: Nihon Kessho Gakkaishi
سال: 1987
ISSN: 0369-4585,1884-5576
DOI: 10.5940/jcrsj.29.232